mock controls Search Results


random flanking sequence control (mock)  (Shanghai GenePharma)
90
Shanghai GenePharma random flanking sequence control (mock)
Random Flanking Sequence Control (Mock), supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mock+controls/random+flanking+sequence+control++mock/pm37495108-177-1-11
Average 90 stars, based on 1 article reviews
random flanking sequence control (mock) - by Bioz Stars, 2026-10
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pcdna4/myc (mock control  (Ji-Feng Biotechnology Co Ltd)
90
Ji-Feng Biotechnology Co Ltd pcdna4/myc (mock control
(A, B) Regulation of COX-2 expression in hepatoma cells is mediated by HCV. pCOX-2-Luc was transfected into Huh7, Ava5, and JFH-1-infected Huh7 cells to measure the effect of COX-2 on HCV protein expression. The cell lysates of Huh7, Ava5 (harboring HCV subgenomic replicon), and JFH-1-infected Huh7 cells were subjected to western blotting. (C) Identification of the viral proteins of HCV that mediate COX-2 regulation. Huh7 cells were co-transfected with pCOX-2-Luc and different viral protein expression vectors (0.5 μg). (D) HCV NS5A induces COX-2 transcriptional activity. Huh7 cells were co-transfected with pCOX-2-Luc and different amounts of pCMV-NS5A-Myc (0–2 μg). After incubation for 3 days, the cell lysates were subjected to the luciferase activity assay to measure the induction of the COX-2 promoter. The activity of Ava5 cells was the positive control. “Mock” indicates the co-transfection of empty vector, <t>pcDNA4/myc,</t> and pCOX-2-Luc. Data shown are mean ± SE; n = 3. *p < 0.05. **p < 0.01.
Pcdna4/Myc (Mock Control, supplied by Ji-Feng Biotechnology Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mock+controls/pcdna4+myc++mock+control/pmc04521820-57-23-31
Average 90 stars, based on 1 article reviews
pcdna4/myc (mock control - by Bioz Stars, 2026-10
90/100 stars
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mock community positive controls  (BEI Resources)
90
BEI Resources mock community positive controls
(A, B) Regulation of COX-2 expression in hepatoma cells is mediated by HCV. pCOX-2-Luc was transfected into Huh7, Ava5, and JFH-1-infected Huh7 cells to measure the effect of COX-2 on HCV protein expression. The cell lysates of Huh7, Ava5 (harboring HCV subgenomic replicon), and JFH-1-infected Huh7 cells were subjected to western blotting. (C) Identification of the viral proteins of HCV that mediate COX-2 regulation. Huh7 cells were co-transfected with pCOX-2-Luc and different viral protein expression vectors (0.5 μg). (D) HCV NS5A induces COX-2 transcriptional activity. Huh7 cells were co-transfected with pCOX-2-Luc and different amounts of pCMV-NS5A-Myc (0–2 μg). After incubation for 3 days, the cell lysates were subjected to the luciferase activity assay to measure the induction of the COX-2 promoter. The activity of Ava5 cells was the positive control. “Mock” indicates the co-transfection of empty vector, <t>pcDNA4/myc,</t> and pCOX-2-Luc. Data shown are mean ± SE; n = 3. *p < 0.05. **p < 0.01.
Mock Community Positive Controls, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mock+controls/mock+community+positive+controls/pm34782020-256-5-11
Average 90 stars, based on 1 article reviews
mock community positive controls - by Bioz Stars, 2026-10
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biotinylated scrambled sequence as the control mock (bio-nc)  (Shanghai GenePharma)
90
Shanghai GenePharma biotinylated scrambled sequence as the control mock (bio-nc)
(A, B) Regulation of COX-2 expression in hepatoma cells is mediated by HCV. pCOX-2-Luc was transfected into Huh7, Ava5, and JFH-1-infected Huh7 cells to measure the effect of COX-2 on HCV protein expression. The cell lysates of Huh7, Ava5 (harboring HCV subgenomic replicon), and JFH-1-infected Huh7 cells were subjected to western blotting. (C) Identification of the viral proteins of HCV that mediate COX-2 regulation. Huh7 cells were co-transfected with pCOX-2-Luc and different viral protein expression vectors (0.5 μg). (D) HCV NS5A induces COX-2 transcriptional activity. Huh7 cells were co-transfected with pCOX-2-Luc and different amounts of pCMV-NS5A-Myc (0–2 μg). After incubation for 3 days, the cell lysates were subjected to the luciferase activity assay to measure the induction of the COX-2 promoter. The activity of Ava5 cells was the positive control. “Mock” indicates the co-transfection of empty vector, <t>pcDNA4/myc,</t> and pCOX-2-Luc. Data shown are mean ± SE; n = 3. *p < 0.05. **p < 0.01.
Biotinylated Scrambled Sequence As The Control Mock (Bio Nc), supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mock+controls/biotinylated+scrambled+sequence+as+the+control+mock++bio+nc+/pm39369033-224-2-15
Average 90 stars, based on 1 article reviews
biotinylated scrambled sequence as the control mock (bio-nc) - by Bioz Stars, 2026-10
90/100 stars
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control sirna for mock  (Ribobio co)
90
Ribobio co control sirna for mock
(A, B) Regulation of COX-2 expression in hepatoma cells is mediated by HCV. pCOX-2-Luc was transfected into Huh7, Ava5, and JFH-1-infected Huh7 cells to measure the effect of COX-2 on HCV protein expression. The cell lysates of Huh7, Ava5 (harboring HCV subgenomic replicon), and JFH-1-infected Huh7 cells were subjected to western blotting. (C) Identification of the viral proteins of HCV that mediate COX-2 regulation. Huh7 cells were co-transfected with pCOX-2-Luc and different viral protein expression vectors (0.5 μg). (D) HCV NS5A induces COX-2 transcriptional activity. Huh7 cells were co-transfected with pCOX-2-Luc and different amounts of pCMV-NS5A-Myc (0–2 μg). After incubation for 3 days, the cell lysates were subjected to the luciferase activity assay to measure the induction of the COX-2 promoter. The activity of Ava5 cells was the positive control. “Mock” indicates the co-transfection of empty vector, <t>pcDNA4/myc,</t> and pCOX-2-Luc. Data shown are mean ± SE; n = 3. *p < 0.05. **p < 0.01.
Control Sirna For Mock, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mock+controls/control+sirna+for+mock/pmc06131627-53-10-20
Average 90 stars, based on 1 article reviews
control sirna for mock - by Bioz Stars, 2026-10
90/100 stars
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mock community a control bma  (BEI Resources)
90
BEI Resources mock community a control bma
(A, B) Regulation of COX-2 expression in hepatoma cells is mediated by HCV. pCOX-2-Luc was transfected into Huh7, Ava5, and JFH-1-infected Huh7 cells to measure the effect of COX-2 on HCV protein expression. The cell lysates of Huh7, Ava5 (harboring HCV subgenomic replicon), and JFH-1-infected Huh7 cells were subjected to western blotting. (C) Identification of the viral proteins of HCV that mediate COX-2 regulation. Huh7 cells were co-transfected with pCOX-2-Luc and different viral protein expression vectors (0.5 μg). (D) HCV NS5A induces COX-2 transcriptional activity. Huh7 cells were co-transfected with pCOX-2-Luc and different amounts of pCMV-NS5A-Myc (0–2 μg). After incubation for 3 days, the cell lysates were subjected to the luciferase activity assay to measure the induction of the COX-2 promoter. The activity of Ava5 cells was the positive control. “Mock” indicates the co-transfection of empty vector, <t>pcDNA4/myc,</t> and pCOX-2-Luc. Data shown are mean ± SE; n = 3. *p < 0.05. **p < 0.01.
Mock Community A Control Bma, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mock+controls/mock+community+a+control+bma/pm40318838-66-21-16
Average 90 stars, based on 1 article reviews
mock community a control bma - by Bioz Stars, 2026-10
90/100 stars
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mock control sictr  (Ribobio co)
90
Ribobio co mock control sictr
(A, B) Regulation of COX-2 expression in hepatoma cells is mediated by HCV. pCOX-2-Luc was transfected into Huh7, Ava5, and JFH-1-infected Huh7 cells to measure the effect of COX-2 on HCV protein expression. The cell lysates of Huh7, Ava5 (harboring HCV subgenomic replicon), and JFH-1-infected Huh7 cells were subjected to western blotting. (C) Identification of the viral proteins of HCV that mediate COX-2 regulation. Huh7 cells were co-transfected with pCOX-2-Luc and different viral protein expression vectors (0.5 μg). (D) HCV NS5A induces COX-2 transcriptional activity. Huh7 cells were co-transfected with pCOX-2-Luc and different amounts of pCMV-NS5A-Myc (0–2 μg). After incubation for 3 days, the cell lysates were subjected to the luciferase activity assay to measure the induction of the COX-2 promoter. The activity of Ava5 cells was the positive control. “Mock” indicates the co-transfection of empty vector, <t>pcDNA4/myc,</t> and pCOX-2-Luc. Data shown are mean ± SE; n = 3. *p < 0.05. **p < 0.01.
Mock Control Sictr, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mock+controls/mock+control+sictr/pm35880565-63-1-8
Average 90 stars, based on 1 article reviews
mock control sictr - by Bioz Stars, 2026-10
90/100 stars
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randomized flanking sequence control (mock)  (Shanghai GenePharma)
90
Shanghai GenePharma randomized flanking sequence control (mock)
(A, B) Regulation of COX-2 expression in hepatoma cells is mediated by HCV. pCOX-2-Luc was transfected into Huh7, Ava5, and JFH-1-infected Huh7 cells to measure the effect of COX-2 on HCV protein expression. The cell lysates of Huh7, Ava5 (harboring HCV subgenomic replicon), and JFH-1-infected Huh7 cells were subjected to western blotting. (C) Identification of the viral proteins of HCV that mediate COX-2 regulation. Huh7 cells were co-transfected with pCOX-2-Luc and different viral protein expression vectors (0.5 μg). (D) HCV NS5A induces COX-2 transcriptional activity. Huh7 cells were co-transfected with pCOX-2-Luc and different amounts of pCMV-NS5A-Myc (0–2 μg). After incubation for 3 days, the cell lysates were subjected to the luciferase activity assay to measure the induction of the COX-2 promoter. The activity of Ava5 cells was the positive control. “Mock” indicates the co-transfection of empty vector, <t>pcDNA4/myc,</t> and pCOX-2-Luc. Data shown are mean ± SE; n = 3. *p < 0.05. **p < 0.01.
Randomized Flanking Sequence Control (Mock), supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mock+controls/randomized+flanking+sequence+control++mock+/10__1074_slash_jbc__ra118__006853-184-2-11
Average 90 stars, based on 1 article reviews
randomized flanking sequence control (mock) - by Bioz Stars, 2026-10
90/100 stars
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negative control sirna s5c-0600  (Cosmo Bio USA)
90
Cosmo Bio USA negative control sirna s5c-0600
(A, B) Regulation of COX-2 expression in hepatoma cells is mediated by HCV. pCOX-2-Luc was transfected into Huh7, Ava5, and JFH-1-infected Huh7 cells to measure the effect of COX-2 on HCV protein expression. The cell lysates of Huh7, Ava5 (harboring HCV subgenomic replicon), and JFH-1-infected Huh7 cells were subjected to western blotting. (C) Identification of the viral proteins of HCV that mediate COX-2 regulation. Huh7 cells were co-transfected with pCOX-2-Luc and different viral protein expression vectors (0.5 μg). (D) HCV NS5A induces COX-2 transcriptional activity. Huh7 cells were co-transfected with pCOX-2-Luc and different amounts of pCMV-NS5A-Myc (0–2 μg). After incubation for 3 days, the cell lysates were subjected to the luciferase activity assay to measure the induction of the COX-2 promoter. The activity of Ava5 cells was the positive control. “Mock” indicates the co-transfection of empty vector, <t>pcDNA4/myc,</t> and pCOX-2-Luc. Data shown are mean ± SE; n = 3. *p < 0.05. **p < 0.01.
Negative Control Sirna S5c 0600, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mock+controls/mock+control+oligonucleotides+s5c+0600/pm22832223-192-0-7
Average 90 stars, based on 1 article reviews
negative control sirna s5c-0600 - by Bioz Stars, 2026-10
90/100 stars
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pcdnatm 6.2-gw/emgfpmir-neg transduced negative control cells (mock)  (clea japan inc)
90
clea japan inc pcdnatm 6.2-gw/emgfpmir-neg transduced negative control cells (mock)
(A, B) Regulation of COX-2 expression in hepatoma cells is mediated by HCV. pCOX-2-Luc was transfected into Huh7, Ava5, and JFH-1-infected Huh7 cells to measure the effect of COX-2 on HCV protein expression. The cell lysates of Huh7, Ava5 (harboring HCV subgenomic replicon), and JFH-1-infected Huh7 cells were subjected to western blotting. (C) Identification of the viral proteins of HCV that mediate COX-2 regulation. Huh7 cells were co-transfected with pCOX-2-Luc and different viral protein expression vectors (0.5 μg). (D) HCV NS5A induces COX-2 transcriptional activity. Huh7 cells were co-transfected with pCOX-2-Luc and different amounts of pCMV-NS5A-Myc (0–2 μg). After incubation for 3 days, the cell lysates were subjected to the luciferase activity assay to measure the induction of the COX-2 promoter. The activity of Ava5 cells was the positive control. “Mock” indicates the co-transfection of empty vector, <t>pcDNA4/myc,</t> and pCOX-2-Luc. Data shown are mean ± SE; n = 3. *p < 0.05. **p < 0.01.
Pcdnatm 6.2 Gw/Emgfpmir Neg Transduced Negative Control Cells (Mock), supplied by clea japan inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mock+controls/pcdnatm+6+2+gw+emgfpmir+neg+transduced+negative+control+cells++mock+/pm22244889-62-0-27
Average 90 stars, based on 1 article reviews
pcdnatm 6.2-gw/emgfpmir-neg transduced negative control cells (mock) - by Bioz Stars, 2026-10
90/100 stars
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flanking sequence control (mock)  (Shanghai GenePharma)
90
Shanghai GenePharma flanking sequence control (mock)
(A, B) Regulation of COX-2 expression in hepatoma cells is mediated by HCV. pCOX-2-Luc was transfected into Huh7, Ava5, and JFH-1-infected Huh7 cells to measure the effect of COX-2 on HCV protein expression. The cell lysates of Huh7, Ava5 (harboring HCV subgenomic replicon), and JFH-1-infected Huh7 cells were subjected to western blotting. (C) Identification of the viral proteins of HCV that mediate COX-2 regulation. Huh7 cells were co-transfected with pCOX-2-Luc and different viral protein expression vectors (0.5 μg). (D) HCV NS5A induces COX-2 transcriptional activity. Huh7 cells were co-transfected with pCOX-2-Luc and different amounts of pCMV-NS5A-Myc (0–2 μg). After incubation for 3 days, the cell lysates were subjected to the luciferase activity assay to measure the induction of the COX-2 promoter. The activity of Ava5 cells was the positive control. “Mock” indicates the co-transfection of empty vector, <t>pcDNA4/myc,</t> and pCOX-2-Luc. Data shown are mean ± SE; n = 3. *p < 0.05. **p < 0.01.
Flanking Sequence Control (Mock), supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mock+controls/flanking+sequence+control++mock+/10__1128_slash_jvi__01022___18-55-2-12
Average 90 stars, based on 1 article reviews
flanking sequence control (mock) - by Bioz Stars, 2026-10
90/100 stars
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mock n-egfp-negative control n-egfp-nc  (Shanghai GenePharma)
90
Shanghai GenePharma mock n-egfp-negative control n-egfp-nc
(A, B) Regulation of COX-2 expression in hepatoma cells is mediated by HCV. pCOX-2-Luc was transfected into Huh7, Ava5, and JFH-1-infected Huh7 cells to measure the effect of COX-2 on HCV protein expression. The cell lysates of Huh7, Ava5 (harboring HCV subgenomic replicon), and JFH-1-infected Huh7 cells were subjected to western blotting. (C) Identification of the viral proteins of HCV that mediate COX-2 regulation. Huh7 cells were co-transfected with pCOX-2-Luc and different viral protein expression vectors (0.5 μg). (D) HCV NS5A induces COX-2 transcriptional activity. Huh7 cells were co-transfected with pCOX-2-Luc and different amounts of pCMV-NS5A-Myc (0–2 μg). After incubation for 3 days, the cell lysates were subjected to the luciferase activity assay to measure the induction of the COX-2 promoter. The activity of Ava5 cells was the positive control. “Mock” indicates the co-transfection of empty vector, <t>pcDNA4/myc,</t> and pCOX-2-Luc. Data shown are mean ± SE; n = 3. *p < 0.05. **p < 0.01.
Mock N Egfp Negative Control N Egfp Nc, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mock+controls/mock+n+egfp+negative+control+n+egfp+nc/pmc04065187-312-7-13
Average 90 stars, based on 1 article reviews
mock n-egfp-negative control n-egfp-nc - by Bioz Stars, 2026-10
90/100 stars
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Image Search Results


(A, B) Regulation of COX-2 expression in hepatoma cells is mediated by HCV. pCOX-2-Luc was transfected into Huh7, Ava5, and JFH-1-infected Huh7 cells to measure the effect of COX-2 on HCV protein expression. The cell lysates of Huh7, Ava5 (harboring HCV subgenomic replicon), and JFH-1-infected Huh7 cells were subjected to western blotting. (C) Identification of the viral proteins of HCV that mediate COX-2 regulation. Huh7 cells were co-transfected with pCOX-2-Luc and different viral protein expression vectors (0.5 μg). (D) HCV NS5A induces COX-2 transcriptional activity. Huh7 cells were co-transfected with pCOX-2-Luc and different amounts of pCMV-NS5A-Myc (0–2 μg). After incubation for 3 days, the cell lysates were subjected to the luciferase activity assay to measure the induction of the COX-2 promoter. The activity of Ava5 cells was the positive control. “Mock” indicates the co-transfection of empty vector, pcDNA4/myc, and pCOX-2-Luc. Data shown are mean ± SE; n = 3. *p < 0.05. **p < 0.01.

Journal: PLoS ONE

Article Title: HCV NS5A Up-Regulates COX-2 Expression via IL-8-Mediated Activation of the ERK/JNK MAPK Pathway

doi: 10.1371/journal.pone.0133264

Figure Lengend Snippet: (A, B) Regulation of COX-2 expression in hepatoma cells is mediated by HCV. pCOX-2-Luc was transfected into Huh7, Ava5, and JFH-1-infected Huh7 cells to measure the effect of COX-2 on HCV protein expression. The cell lysates of Huh7, Ava5 (harboring HCV subgenomic replicon), and JFH-1-infected Huh7 cells were subjected to western blotting. (C) Identification of the viral proteins of HCV that mediate COX-2 regulation. Huh7 cells were co-transfected with pCOX-2-Luc and different viral protein expression vectors (0.5 μg). (D) HCV NS5A induces COX-2 transcriptional activity. Huh7 cells were co-transfected with pCOX-2-Luc and different amounts of pCMV-NS5A-Myc (0–2 μg). After incubation for 3 days, the cell lysates were subjected to the luciferase activity assay to measure the induction of the COX-2 promoter. The activity of Ava5 cells was the positive control. “Mock” indicates the co-transfection of empty vector, pcDNA4/myc, and pCOX-2-Luc. Data shown are mean ± SE; n = 3. *p < 0.05. **p < 0.01.

Article Snippet: To evaluate the regulation of COX-2 and IL-8 expressions by NS5A, Huh7 and Ava5 cells were transfected with different concentrations of pCMV-NS5A-Myc or control vector pcDNA4/myc (mock control) by T-Pro reagent (Ji-Feng Biotechnology Co., Ltd. Taiwan) according to the manufacturer’s instructions.

Techniques: Expressing, Transfection, Infection, Western Blot, Activity Assay, Incubation, Luciferase, Positive Control, Cotransfection, Plasmid Preparation